Sub-Cellular Motion Compensation for Minimally Invasive Microscopy, in vivo: Evidence for Oxygen Gradients in Resting Muscle – Online Supplement
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چکیده
Carbomer gel was prepared with 300mM D-sorbitol (Sigma-Aldrich, St. Louis, MO) and 10mM HEPES free acid (Sigma-Aldrich, St. Louis, MO) in heated distilled water. A gelling agent, carbomer 940 (Snowdrift Farm, Tucson, AZ), was added to the heated sorbitol solution under constant stirring, to reach a concentration of 0.3%, based on a weight percent. Once the carbomer was completely dissolved in the solution, pH was adjusted by drop-wise addition of triethanolamine to 7.2-7.4. The gel was stored in a sealed container at 4°C.
منابع مشابه
Short communication: Subcellular motion compensation for minimally invasive microscopy, in vivo: evidence for oxygen gradients in resting muscle.
RATIONALE In vivo microscopy seeks to observe dynamic subcellular processes in a physiologically relevant context. A primary limitation of optical microscopy in vivo is tissue motion, which prevents physiological time course observations or image averaging. OBJECTIVE To develop and demonstrate motion compensation methods that can automatically track image planes within biological tissues, inc...
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